Product Overview
Description:
AdipoUpX™ Human APOE MSCV Particle (Overexpression) is a powerful gene delivery tool designed for the efficient, stable overexpression of the human APOE gene. APOE is critical for the transport and metabolism of cholesterol and other lipids. Its MSCV backbone is optimized for hematopoietic stem cells, stem cells, etc., providing a robust platform for studying the genetic drivers of cardiovascular disease and obesity. All products undergo rigorous testing, including titer and mycoplasma detection, ensuring the viral system is both high-quality and effective for long-term functional studies.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
APOE
Full Name:
Apolipoprotein E
Gene Symbol:
AD2; LPG; APO-E; ApoE4; LDLCQ5
Summary:
APOE encodes a major apolipoprotein of chylomicrons, critical for binding liver and peripheral receptors and facilitating the clearance of triglyceride-rich lipoproteins. Located on chromosome 19 alongside APOC1 and APOC2, APOE mutations cause familial dysbetalipoproteinemia (type III hyperlipoproteinemia), leading to elevated plasma cholesterol and triglycerides due to impaired clearance of chylomicron and VLDL remnants.
