AdipoUpX™ Human ENPP2 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ENPP2 MSCV Particle (Overexpression) is engineered for long-term overexpression of the human ENPP2 gene, encoding autotaxin, a regulator of lysophosphatidic acid signaling. ENPP2 is implicated in adipose tissue expansion, inflammation, and metabolic disease. This MSCV retroviral system supports stable expression in stem cells, hematopoietic stem cells, and other mammalian cell lines. Each product is supported by comprehensive QC testing to ensure safety and reproducibility.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: ENPP2

Full Name: Ectonucleotide pyrophosphatase/phosphodiesterase 2

Gene Symbol: ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA

Gene ID: 5168

RefSeq ID-1: NP_001035181.1

RefSeq ID-2: NM_001040092.3

Summary: The ENPP2 gene produces Autotaxin, a secreted enzyme that generates lysophosphatidic acid (LPA), a potent signaling lipid. LPA triggers growth-factor-like responses, including cell migration and proliferation. In the context of metabolism, Autotaxin expression is often upregulated in adipose tissue during obesity, where it may contribute to the inflammation and remodeling of fat depots.

AdipoUpX™ Human ENPP2 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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