Product Overview
Description:
AdipoUpX™ Human INS MSCV Particle (Overexpression) is a high-titer MSCV particle professionally engineered for the specific and efficient stable expression of the human INS (Insulin) gene. The MSCV system is uniquely suited for driving strong gene integration in hematopoietic stem cells, stem cells, etc., making it an ideal choice for establishing stable lines to study insulin biosynthesis and secretion. This ready-to-use tool ensures consistent performance across various mammalian lineages. Comprehensive quality control screening ensures the safety, efficacy, and purity of every viral product.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
INS
Full Name:
Insulin
Gene Symbol:
IDDM; ILPR; IRDN; IDDM1; IDDM2; PNDM4; MODY10
Summary:
INS is the gene responsible for the production of insulin, a central hormone governing glucose utilization and lipid metabolism. Translation of the INS transcript yields a precursor protein that is sequentially processed to proinsulin and ultimately cleaved into mature insulin and C-peptide. The biologically active insulin molecule consists of two peptide chains connected by disulfide bonds and exerts its effects through binding to the insulin receptor, thereby stimulating glucose uptake into target tissues. Numerous disease-associated INS mutations have been identified, contributing to diverse metabolic phenotypes such as neonatal diabetes, MODY type 10, and abnormal proinsulin processing. The INS locus overlaps with the INS-IGF2 read-through transcript, reflecting complex transcriptional architecture.
