AdipoUpX™ Human MC4R MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human MC4R MSCV Particle (Overexpression) is a professionally engineered vector particle providing efficient and stable overexpression of human MC4R. The vector is optimized specifically for hematopoietic cells and stem cells. This makes it an ideal tool for establishing stable cell lines to investigate the central hypothalamic MC4R GPCR. Overexpressing MC4R facilitates investigation of its downstream signaling mechanisms and role in energy balance for drug screening research. The viral system features a modular design offering high flexibility.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: MC4R

Full Name: Melanocortin receptor 4

Gene Symbol: BMIQ20

Gene ID: 4160

RefSeq ID: NM_005912.3, NP_005903.2

Summary: The protein encoded by the MC4R gene is a key receptor in the hypothalamic appetite regulation pathway, belonging to the G protein-coupled receptor (GPCR) family. This receptor activates intracellular signaling by binding to ligands such as POMC cleavage products (α-MSH), thereby producing physiological effects that suppress appetite. MC4R functional deficiency or mutation is the primary cause of monogenic obesity in humans. The gene's unique intronless structure means its mRNA coding region is continuous, simplifying gene expression and research models.

AdipoUpX™ Human MC4R MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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