Product Overview
Description:
AdipoUpX™ Human ATG7 MSCV Particle (Overexpression) is engineered for stable expression of the human ATG7 gene, an essential autophagy-related enzyme. Autophagy plays a critical role in adipocyte function, lipid turnover, and obesity-associated metabolic adaptation. The MSCV system enables robust expression in stem cells and diverse mammalian cell lines. Each product is validated through strict QC testing.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
ATG7
Full Name:
Autophagy related 7
Gene Symbol:
GSA7; APG7L; SCAR31; APG7-LIKE
Summary:
ATG7 encodes an essential autophagy enzyme required for cellular recycling and metabolic adaptation. In adipose tissue, ATG7 regulates adipocyte differentiation, lipid turnover, and mitochondrial quality control. Impaired ATG7 function disrupts metabolic homeostasis and promotes obesity-related phenotypes. Several transcript variants are expressed.
