AdipoUpX™ Human ACSL4 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ACSL4 MSCV Particle (Overexpression) is a stable gene delivery tool for the overexpression of the human ACSL4 gene, a long-chain acyl-CoA synthetase involved in fatty acid activation and lipid remodeling. ACSL4 activity influences lipid partitioning and metabolic stress associated with obesity. The MSCV vector enables high-level expression in hematopoietic stem cells, stem cells, and additional mammalian systems. Each lot is subjected to full quality control, including titer validation and microbial safety testing.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: ACSL4

Full Name: Acyl-CoA synthetase long chain family member 4

Gene Symbol: ACS4; FACL4; LACS4; MRX63; MRX68; XLID63

Gene ID: 2182

RefSeq ID-1: NP_001305438.1

RefSeq ID-2: NM_001318509.2

Summary: ACSL4 is a specialized isozyme in the long-chain fatty-acid-CoA ligase family, showing a high preference for arachidonate as a substrate. It converts free fatty acids into fatty acyl-CoA esters, a step required for both the synthesis of new lipids and the degradation of existing fats. Because it handles specific polyunsaturated fatty acids, its activity is vital for membrane composition and inflammatory signaling.

AdipoUpX™ Human ACSL4 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

Related Products