Product Overview
Description:
AdipoUpX™ Human PIK3CA MSCV Particle (Overexpression) is a high-titer MSCV particle designed for the specific and efficient stable overexpression of the human PIK3CA gene. As a key component of the PI3K/AKT pathway, it is essential for insulin signaling and glucose transport. This system is optimized for stem cells and various mammalian cell types, ensuring robust expression for investigating insulin sensitivity. The viral system features a modular design for high flexibility, while comprehensive quality control screening ensures the highest performance and safety standards.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
PIK3CA
Full Name:
Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Gene Symbol:
HMH; MCM; CCM4; CWS5; MCAP; PI3K; CLAPO; CLOVE; MCMTC; PI3K-alpha; p110-alpha
Summary:
PIK3CA encodes the catalytic subunit of phosphatidylinositol 3-kinase (PI3K), which phosphorylates phosphoinositides using ATP. This kinase plays a central role in intracellular signaling pathways, including insulin signaling and cell growth. Although primarily studied in cancer, dysregulation of PIK3CA can also influence energy metabolism. A pseudogene is located on chromosome 22.
