AdipoUpX™ Human LEPR MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human LEPR MSCV Particle (Overexpression) is a stable integration tool for the specific and efficient overexpression of the human LEPR gene. The vector is optimized for hematopoietic cells, ensuring stable expression. LEPR is the key entry point for LEP signaling. This product allows systematic investigation into leptin resistance and downstream JAK/STAT activation. It serves as a powerful tool for in vitro functional validation and signaling studies. The product is ready-to-use, and all viral systems undergo rigorous testing to ensure quality.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: LEPR

Full Name: Leptin receptor

Gene Symbol: OBR; OB-R; CD295; LEP-R; LEPRD

Gene ID: 3953

RefSeq ID: NM_001003679.3, NP_001003679.1

Summary: The protein encoded by the LEPR gene serves as the terminal receptor for leptin signaling, functioning as a transmembrane cytokine receptor. Its core mechanism involves activating cytoplasmic JAK kinases upon leptin binding, which subsequently phosphorylates STAT proteins and facilitates their nuclear translocation to regulate target gene expression and influence energy metabolism. LEPR serves not only as a central regulator of adipose and energy homeostasis but also participates in normal lymphopoiesis. Functional defects in LEPR disrupt leptin signaling, leading to severe obesity often accompanied by endocrine complications such as pituitary dysfunction.

AdipoUpX™ Human LEPR MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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