Product Overview
Description:
AdipoUpX™ Human KIDINS220 MSCV Particle (Overexpression) is a professionally engineered vector particle providing efficient and stable overexpression of human KIDINS220. This scaffold protein is essential for neurotrophin signaling and has been linked to severe early-onset obesity. The MSCV system is uniquely suited for driving strong gene integration in hematopoietic stem cells, stem cells, and other mammalian cell lines, facilitating the study of downstream signaling mechanisms. Every batch is validated through strict quality control to ensure repeatability.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
KIDINS220
Full Name:
Kinase D interacting substrate 220
Gene Symbol:
ARMS; SINO; VENARG
Summary:
KIDINS220 encodes a transmembrane scaffold protein highly expressed in the nervous system. It regulates neuronal survival, dendrite and axon development, and synaptic plasticity by mediating interactions among membrane receptors, signaling proteins, and the cytoskeleton. Mutations are associated with spastic paraplegia, intellectual disability, nystagmus, obesity, and neurodegenerative diseases, including Alzheimer’s. Multiple transcript variants arise through alternative splicing.
