AdipoUpX™ Human FTO MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human FTO MSCV Particle (Overexpression) is a powerful gene delivery tool designed for the stable overexpression of the human FTO gene. The FTO protein is a key target in studying nucleic acid demethylation and fat accumulation regulation. Its structure is optimized for hematopoietic cells and stem cells, providing stable gene integration. This product accelerates progress in obesity genetics by enabling rapid validation of FTO's effects on energy expenditure and metabolic signaling pathways. All products undergo rigorous testing to ensure quality and efficacy.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: FTO

Full Name: Alpha-ketoglutarate-dependent dioxygenase

Gene Symbol: GDFD; IFEX9; ALKBH9; BMIQ14

Gene ID: 79068

RefSeq ID: NM_001080432.3, NP_001073901.1

Summary: The FTO gene encodes a nuclear-localized Fe(II)- and 2-ketoglutarate-dependent oxygenase. As a member of the AlkB family, it possesses oxidative demethylation catalytic activity to repair alkylating damage in DNA and RNA. Common single-nucleotide polymorphisms (SNPs) in the FTO gene are positively correlated with body mass index (BMI) and obesity risk. Functional studies indicate FTO protein expression in neural and cardiovascular systems, suggesting its critical role in cross-system energy balance and metabolic regulation.

AdipoUpX™ Human FTO MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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