Product Overview
Description:
AdipoUpX™ Human TLR4 MSCV Particle (Overexpression) is a high-titer retroviral tool designed for stable overexpression of the human TLR4 gene. TLR4 is a key innate immune receptor linking inflammation to metabolic dysfunction in obesity. The MSCV vector enables durable expression in hematopoietic stem cells, stem cells, and mammalian models. Each batch undergoes sterility, titer, and mycoplasma testing.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
TLR4
Full Name:
Toll like receptor 4
Gene Symbol:
TOLL; CD284; TLR-4; ARMD10
Summary:
TLR4 encodes a pattern-recognition receptor of the Toll-like receptor family, which is central to innate immune activation. It detects pathogen-associated molecular patterns, particularly lipopolysaccharide from gram-negative bacteria, triggering downstream inflammatory signaling and cytokine production. TLR4 is widely expressed but shows tissue-specific regulation. Variants of this receptor influence responsiveness to bacterial components and have been associated with altered inflammatory susceptibility. It has also been studied in the context of viral protein interactions and immune modulation.
