Product Overview
Description:
AdipoUpX™ Human LEP MMLV Particle (Overexpression) is a high-titer, ready-to-use MMLV retroviral particle. It is professionally engineered for the efficient and stable overexpression of the human LEP gene. The MMLV vector stably integrates into the genome of dividing host cells, making it an ideal tool for establishing stable cell lines. This product is particularly suitable for in-depth research in obesity, endocrinology, and energy homeostasis. The viral system features a modular design, allowing high flexibility in customizing functional components. All products undergo comprehensive quality control, including titer, sterility testing, and mycoplasma detection.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.
Sterility Test:
The final viral preparation was confirmed sterile after testing.
Mycoplasma Test:
Mycoplasma contamination testing met all release specifications for all product lots.
Other QC:
We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.
Storage:
The product must be immediately transferred to -80 °C storage upon its arrival.
Stability:
For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.
Shipping Condition:
Our MMLV retroviral products are shipped using dry ice.
Handling Notes:
Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.
Target Profile
Gene Name:
LEP
Full Name:
Leptin
Gene Symbol:
OBS; OB; LEPD
Summary:
LEP (leptin) protein is a cytokine-like hormone derived from adipocytes that primarily acts on the central nervous system to maintain energy balance. Its molecular mechanism involves binding to receptors in the brain, triggering the activation of downstream signaling pathways such as JAK-STAT, thereby signaling satiety, reducing food intake, and increasing basal metabolism. Beyond weight regulation, LEP's endocrine effects extend to immune, reproductive, hematopoietic, and wound healing processes. Mutations in the LEP gene or its regulatory elements cause severe, early-onset obesity in humans, often accompanied by hypogonadism and type 2 diabetes complications.
