AdipoUpX™ Human LEPR MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human LEPR MMLV Particle (Overexpression) is a stable integration tool for the specific and efficient overexpression of the human LEPR gene. LEPR serves as the key entry point for the LEP signaling pathway. This ready-to-use product allows systematic investigation into leptin resistance and downstream JAK/STAT activation. It is an ideal tool for in vitro functional validation and signaling studies in dividing host cells. Product quality is ensured by rigorous purification, titer validation, and comprehensive QC screening against contaminants.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: LEPR

Full Name: Leptin receptor

Gene Symbol: OBR; OB-R; CD295; LEP-R; LEPRD

Gene ID: 3953

RefSeq ID: NM_001003679.3, NP_001003679.1

Summary: The protein encoded by the LEPR gene serves as the gateway for leptin signaling, functioning as a transmembrane cytokine receptor. Its primary molecular mechanism involves binding to leptin to activate intracellular JAK-STAT signaling pathways, directly regulating gene expression in target cells (such as hypothalamic neurons) to control adipose tissue mass and energy expenditure. LEPR serves not only as a central metabolic receptor but also participates in hematopoietic pathways essential for T cell development. Functional mutations in the LEPR gene are associated with human obesity and certain types of pituitary dysfunction.

AdipoUpX™ Human LEPR MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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