Product Overview
Description:
AdipoUpX™ Human MAP3K8 MMLV Particle (Overexpression) is a high-titer, ready-to-use MMLV retroviral particle professionally engineered for the stable overexpression of the human MAP3K8 gene. MAP3K8 is a MAP kinase kinase kinase involved in inflammatory signaling pathways that intersect with insulin resistance and metabolic stress responses. Stable integration enables long-term studies of inflammation-driven metabolic dysfunction in dividing cells. Each batch undergoes rigorous quality control, including titer, sterility, and mycoplasma testing.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.
Sterility Test:
The final viral preparation was confirmed sterile after testing.
Mycoplasma Test:
Mycoplasma contamination testing met all release specifications for all product lots.
Other QC:
We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.
Storage:
The product must be immediately transferred to -80 °C storage upon its arrival.
Stability:
For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.
Shipping Condition:
Our MMLV retroviral products are shipped using dry ice.
Handling Notes:
Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.
Target Profile
Gene Name:
MAP3K8
Full Name:
Mitogen-activated protein kinase kinase kinase 8
Gene Symbol:
COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT
Summary:
MAP3K8 encodes a cytoplasmic serine/threonine kinase that functions as a signaling mediator within MAPK and JNK pathways. It can activate IκB kinases, leading to downstream NF-κB signaling and transcriptional activation of inflammatory genes. The protein also contributes to cytokine production, including TNF-α and IL-2, during T-cell activation. In addition to the full-length form, an alternative translation initiation site may generate a shorter isoform with reduced transforming activity. Multiple transcript variants have been reported.
