AdipoUpX™ Human ACSL4 MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ACSL4 MMLV Particle (Overexpression) is a high-titer MMLV particle designed for the efficient and stable overexpression of the human ACSL4 gene. This enzyme is vital for fatty acid partitioning and has been linked to ferroptosis and lipid metabolism in metabolic syndromes. The MMLV system’s ability to integrate into the host genome makes it a perfect tool for investigating lipid flux in stable cell models. The viral system features a modular design for high flexibility, and all products undergo comprehensive quality control to ensure reproducibility.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: ACSL4

Full Name: Acyl-CoA synthetase long chain family member 4

Gene Symbol: ACS4; FACL4; LACS4; MRX63; MRX68; XLID63

Gene ID: 2182

RefSeq ID-1: NP_001305438.1

RefSeq ID-2: NM_001318509.2

Summary: ACSL4 encodes a long-chain fatty acid-CoA ligase isozyme, preferentially activating arachidonic acid. It contributes to lipid biosynthesis and fatty acid degradation. Dysregulation of ACSL4 activity may impact fatty acid metabolism in adipocytes, influencing obesity risk. Multiple transcript variants are generated through alternative splicing.

AdipoUpX™ Human ACSL4 MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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