AdipoUpX™ Human FTO MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human FTO MMLV Particle (Overexpression) is a powerful gene delivery tool designed for the stable overexpression of the human FTO gene. The FTO protein is a key target in studying nucleic acid demethylation and fat accumulation regulation. Due to its stable integration into dividing cell genomes, this MMLV particle is perfect for accelerating stable cell line establishment. It enables rapid validation of FTO's effects on energy expenditure and metabolic signaling pathways in cellular models. Clients can freely customize key functional components like promoters and markers.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: FTO

Full Name: Alpha-ketoglutarate-dependent dioxygenase

Gene Symbol: GDFD; IFEX9; ALKBH9; BMIQ14

Gene ID: 79068

RefSeq ID: NM_001080432.3, NP_001073901.1

Summary: The FTO gene encodes a nuclear dioxygenase belonging to the AlkB family, whose activity depends on non-heme iron and 2-oxoglutarate. The enzyme's core molecular function involves nucleic acid demethylation, particularly m6A demethylation in RNA, thereby influencing gene expression and RNA processing. In clinical studies, genetic variants in FTO represent one of the strongest independent genetic predictors of elevated BMI and obesity risk. Furthermore, the extensive expression of FTO in neural and cardiovascular systems suggests its involvement in multiple physiological processes beyond metabolic regulation.

AdipoUpX™ Human FTO MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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