Product Overview
Description:
AdipoUpX™ Human CUL4B MMLV Particle (Overexpression) is a high-titer, ready-to-use MMLV retroviral particle professionally engineered for the stable overexpression of the human CUL4B gene. CUL4B is involved in ubiquitin-mediated protein degradation and epigenetic regulation. Its stable expression supports studies of transcriptional and metabolic control in obesity-related pathways. Each batch undergoes strict QC, including titer, sterility, and mycoplasma testing.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.
Sterility Test:
The final viral preparation was confirmed sterile after testing.
Mycoplasma Test:
Mycoplasma contamination testing met all release specifications for all product lots.
Other QC:
We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.
Storage:
The product must be immediately transferred to -80 °C storage upon its arrival.
Stability:
For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.
Shipping Condition:
Our MMLV retroviral products are shipped using dry ice.
Handling Notes:
Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.
Target Profile
Gene Name:
CUL4B
Full Name:
Cullin 4B
Gene Symbol:
SFM2; MRXSC; CUL-4B; MRXHF2; MRXS15
Summary:
CUL4B encodes a scaffold protein that assembles with partner factors to form a cullin-RING E3 ubiquitin ligase complex. This complex mediates targeted polyubiquitination and subsequent degradation of specific regulatory proteins, including key factors controlling DNA replication, such as chromatin licensing components and cyclin E. Multiple isoforms generated through alternative splicing have been reported.
