AdipoUpX™ Human LIPE MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human LIPE MMLV Particle (Overexpression) is a powerful gene delivery tool designed for the stable overexpression of the human LIPE (HSL) gene. Hormone-sensitive lipase is the key enzyme regulating the mobilization of fatty acids from stored triacylglycerols. The MMLV system’s ability to integrate into the genome makes it ideal for studying the control of lipolysis in stable adipocyte models. Clients can customize promoters and markers, while comprehensive quality control is performed to ensure product safety and high-fidelity results.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: LIPE

Full Name: Lipase E, hormone sensitive type

Gene Symbol: HSL; LHS; REH; AOMS4; FPLD6

Gene ID: 3991

RefSeq ID-1: NP_001403032.1

RefSeq ID-2: NM_001416103.1

Summary: LIPE encodes the Hormone-Sensitive Lipase (HSL), a crucial enzyme in lipid mobilization. This protein exists in a long and a short isoform, generated through the use of alternative translational start codons. The long form is highly expressed in steroidogenic tissues (like the testis), where it hydrolyzes cholesteryl esters to provide free cholesterol for hormone synthesis. The short form is highly expressed in adipose tissue, among other sites, where its primary function is to hydrolyze stored triglycerides into free fatty acids and glycerol, making it a key regulator of systemic energy supply during fasting or stress.

AdipoUpX™ Human LIPE MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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