AdipoUpX™ Human PDE3B MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PDE3B MMLV Particle (Overexpression) is a stable integration tool for the specific and efficient overexpression of the human PDE3B gene. It is a key mediator of insulin's antilipolytic effect, making it a critical focus for studying the regulation of fat mobilization. By integrating stably into the dividing host cell genome, it facilitates the longitudinal study of cAMP signaling and its systemic metabolic consequences. Product quality is ensured by rigorous purification and comprehensive QC screening, guaranteeing safe and repeatable experimental outcomes.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: PDE3B

Full Name: Phosphodiesterase 3B

Gene Symbol: HcGIP1; cGIPDE1

Gene ID: 5140

RefSeq ID-1: NP_000913.2

RefSeq ID-2: NM_000922.3

Summary: PDE3B breaks down cAMP, a vital signaling molecule. By doing so, it acts as a "brake" on the breakdown of fats (lipolysis). Because it is regulated by insulin, PDE3B is the primary reason why insulin stops fat-burning and promotes fat storage after a meal.

AdipoUpX™ Human PDE3B MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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