AdipoUpX™ Human AFF4 MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human AFF4 MMLV Particle (Overexpression) is a high-titer, ready-to-use MMLV retroviral particle professionally engineered for the stable overexpression of the human AFF4 gene. As a component of the super elongation complex, its stable integration provides a reliable platform for researching the transcriptional regulation of adipogenesis and developmental metabolic pathways. Our vector system supports flexible customization of functional components. Every batch undergoes comprehensive quality control to ensure both product safety and the reproducibility of results.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: AFF4

Full Name: ALF transcription elongation factor 4

Gene Symbol: MCEF; CHOPS; AF5Q31

Gene ID: 27125

RefSeq ID-1: NP_055238.1

RefSeq ID-2: NM_014423.4

Summary: AFF4 encodes a transcription-associated protein within the AF4 family and functions as an integral component of the positive transcription elongation factor b (P-TEFb) complex. Through its role in transcriptional elongation, AFF4 regulates gene expression programs involved in cell proliferation and differentiation. Chromosomal rearrangements involving AFF4 and the MLL gene on chromosome 11 have been identified in infant acute lymphoblastic leukemia, underscoring its importance in transcriptional regulation and disease development.

AdipoUpX™ Human AFF4 MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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