AdipoUpX™ Human ACOT11 MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ACOT11 MMLV Particle (Overexpression) is a high-titer MMLV particle designed for the efficient and stable overexpression of the human ACOT11 gene. This thioesterase regulates the balance between fatty acid oxidation and storage, acting as a "brake" on thermogenesis. The MMLV system's stable integration into dividing cells makes it an ideal tool for investigating the molecular drivers of metabolic efficiency in permanent cell lines. The viral system features a modular design for customization and passes rigorous quality control for titer and sterility.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: ACOT11

Full Name: Acyl-CoA thioesterase 11

Gene Symbol: BFIT; THEA; THEM1; STARD14

Gene ID: 26027

RefSeq ID-1: NP_056362.1

RefSeq ID-2: NM_015547.4

Summary: ACOT11 is an enzyme that breaks down activated fatty acids. In mouse models, it is induced by cold temperatures and is found at higher levels in mice that are naturally resistant to obesity. This suggests that ACOT11 acts as a regulator of metabolic "heat" and energy expenditure.

AdipoUpX™ Human ACOT11 MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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