AdipoUpX™ Human POMC MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human POMC MMLV Particle (Overexpression) is a high-titer MMLV particle designed for the efficient and stable overexpression of the human POMC gene. POMC generates key peptides like α-MSH, involved in satiety signaling. This MMLV system's stable integration into dividing cells makes it an ideal tool for investigating hypothalamic neuropeptide metabolism mechanisms and establishing cell models. Overexpressing POMC enables researchers to study its post-translational modifications and precise role in appetite suppression pathways. The viral system offers high flexibility for customization.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: POMC

Full Name: Pro-opiomelanocortin

Gene Symbol: LPH; MSH; NPP; POC; ACTH; CLIP; OBAIRH

Gene ID: 5443

RefSeq ID: NM_000939.4, NP_000930.1

Summary: The POMC gene encodes a large precursor polypeptide characterized by complex tissue-specific post-translational processing. Site-specific cleavage by prohormone convertases releases up to ten biologically active peptides. These products include the key appetite suppressant α-MSH, endorphins, and ACTH. POMC expression and processing exhibit significant differences between the pituitary gland (primarily producing ACTH) and the hypothalamus (primarily producing appetite-regulating peptides). Mutations in the POMC gene clinically result in early-onset severe obesity and associated adrenal insufficiency.

AdipoUpX™ Human POMC MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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