Product Overview
Description:
AdipoUpX™ Human LDLR MMLV Particle (Overexpression) is a high-titer retroviral vector designed for stable overexpression of the human LDLR gene. LDLR is central to cholesterol uptake and lipid homeostasis, making it highly relevant to metabolic disorders, including obesity and dyslipidemia. Stable expression enables functional lipid metabolism studies in cell systems. Each batch is QC-validated for reproducibility.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.
Sterility Test:
The final viral preparation was confirmed sterile after testing.
Mycoplasma Test:
Mycoplasma contamination testing met all release specifications for all product lots.
Other QC:
We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.
Storage:
The product must be immediately transferred to -80 °C storage upon its arrival.
Stability:
For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.
Shipping Condition:
Our MMLV retroviral products are shipped using dry ice.
Handling Notes:
Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.
Target Profile
Gene Name:
LDLR
Full Name:
Low density lipoprotein receptor
Gene Symbol:
LDLCQ2
Summary:
LDLR encodes a membrane-associated receptor responsible for the uptake of circulating low-density lipoprotein particles through receptor-mediated endocytosis. Following internalization, cholesterol is released from lysosomes and participates in feedback regulation of lipid metabolism by suppressing HMG-CoA reductase activity while simultaneously promoting cholesterol ester formation. Loss-of-function variants are a well-established cause of familial hypercholesterolemia, an autosomal dominant lipid disorder. Multiple transcript isoforms arise through alternative splicing.
