Product Overview
Description:
AdipoUpX™ Human POMC Ad5 Adenoviral Particle (Overexpression) is specifically designed for efficient transient overexpression. It enables researchers to study the POMC precursor protein, which generates satiety signaling peptides like α-MSH. This accelerates functional validation of its post-translational modifications and precise role in appetite suppression pathways in cellular models. High customization via reporter genes and comprehensive quality control are integrated features of this powerful tool.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type5 (dE1/E3)
Promoter:
CMV
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations have been validated via PCR to confirm the integrity of their vector genomes.
Sterility Test:
This product was confirmed to be sterile upon testing.
Mycoplasma Test:
This product has passed quality control, confirming that it is free of mycoplasma contamination.
Other QC:
We perform customized supplementary testing and in vitro/in vivo transduction assessments based on specific project requirements to analyze the expression levels and biological functions of transgenes.
Storage:
Upon receipt of viral preparations, they must be immediately transferred to a -80°C environment for long-term storage.
Stability:
The product guarantees excellent stability for 6-12 months when stored continuously at -80°C (and in some cases, up to 2 years). Upon thawing, the working solution is refrigerated at 4°C for up to 2-3 weeks without significant loss of biological activity.
Shipping Condition:
Our adenovirus viral products are shipped using dry ice.
Handling Notes:
Viral particles are highly sensitive to temperature fluctuations and freeze-thaw cycles. To maintain titer and avoid repeated freeze-thaw cycles, it is recommended to aliquot the vector immediately upon receipt into low-protein-binding tubes and store according to specified conditions. Ensure all operations are performed within a biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Our company is committed to product quality, and all products have undergone rigorous internal QC inspections. Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application. Users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements throughout all operational processes.
Target Profile
Gene Name:
POMC
Full Name:
Pro-opiomelanocortin
Gene Symbol:
LPH; MSH; NPP; POC; ACTH; CLIP; OBAIRH
Summary:
This gene encodes a preproprotein that undergoes extensive, tissue-specific post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. The preproprotein contains eight potential cleavage sites, and depending on the tissue type and the specific convertases available, processing can yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is primarily synthesized in the corticotroph cells of the anterior pituitary, where four cleavage sites are utilized; the major end products are adrenocorticotrophin, which is essential for normal steroidogenesis and maintenance of adrenal weight, and beta-lipotropin. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, resulting in peptides that play roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These products include several distinct melanotropins, lipotropins, and endorphins, which are contained within the adrenocorticotrophin and beta-lipotropin peptides. Additionally, the antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early-onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have also been described.
