CEP19 Gene Test Service
InquiryOverview
Obesity is a complex disease caused by environmental factors such as nutrition and genetic factors. Protheragen provides an in-depth explanation of the biological mechanisms of obesity by comprehensively analyzing Obesity Causation and obesity susceptibility gene variants. Mutations in the CEP19 gene are critically linked to morbid obesity and spermatogenic failure. Our lab provides high-quality Monogenic Obesity Gene Test and Obesity Gene Panel Test services.
Explore the Secrets of the CEP19 Gene and Obesity with Us
Our company combines genomics and high-throughput sequencing to provide our clients with accurate Genetically Induced Obesity-related gene testing services.
Genetic Engineering
- Relying on the biological information provided by our clients, our researchers offer professional gene editing modeling services. We provide CEP19 gene knockout or knockdown service to evaluate the mechanistic effects of this gene on obesity in animals.
Transcriptome Sequencing of Adipose Tissue
Our company provides a one-stop transcriptome analysis service to advance our clients' genetic obesity programs.
- Based on highly efficient second-generation sequencing technology, our researchers provide information on the sum of all transcript information transcribed from obese tissues and cells in a specific state.
- Importantly, our company provides coding DNA library construction, sequencing, bioinformatics analysis, and information pathway analysis services.
- Our researchers analyze the transcriptional and regulatory mechanisms in depth by studying the changes in CEP19 gene expression while analyzing the role of DNA coding in its regulation.
Sample Collection
Our company supports sample collection and testing services from various sources, such as tissue and blood. We have specialized bio-researchers to provide sample pre-processing services, including lysis, digestion, centrifugation, decontamination, and purity testing.
Sequencing
Our researchers design specific sequencing primers or probes for the CEP19 gene. We enrich the target gene by polymerase chain reaction (PCR) amplification or other methods and then sequence it using high-throughput sequencing technology. Our company provides clients with accurate detection of sequence variants in genes.
Data Processing
Our researchers compare reference databases to determine whether there are new or known mutations in the CEP19 gene. For the large amount of data obtained from sequencing, our company has a professional bioinformatics team to provide efficient data processing services.
Workflow
Applications
- The CEP19 gene test provides comprehensive, accurate, and scientific data support for preclinical studies of single-gene genetic diseases.
- The CEP19 gene test can be used as an effective target to prevent and treat the development of obesity.
- The CEP19 gene test provides a database for studying the mechanisms of inflammation in adipose tissue.
Advantages of Us
- Our company has a standardized genetic analysis process and skilled bioresearchers to accelerate your project.
- In genetic analysis, our company supports the screening of genes, customization of parameters, and personalized charting.
- We possess senior bioinformatics experts who excel in programming and integrating diverse bioinformatics analysis software, possessing the expertise to conduct in-depth mining and comprehensive analysis of extensive sequencing data and its associated databases.
Your Best Anti-Obesity Research Assistant
Publication Data
Technology: Gene editing, Cell modeling, Super-resolution microscopy, Imaging, Western blot, Electron microscopy, Clustered regularly interspaced short palindromic repeats (CRISPR)
Journal: Open Biology
Published: 2017
Results: The researchers used CRISPR technology to knock out the CEP19 gene to observe its effect on the ability to form cilia and compare it to the knockout effects of FGFR1OP (FOP) and CEP350. Ciliogenesis and mutations contribute in part to morbid obesity. The ability of CEP19 CRISPR knockout cells to form cilia was severely impaired, similar to the loss of function of CEP19's binding partners, FOP and CEP350. In the absence of CEP19, microtubules were anchored in the centrosome like that in the absence of FOP and CEP350, suggesting that the CEP19 gene plays a critical role in microtubule anchoring.
Fig.1 Effect of the CEP19 gene on the early stages of ciliogenesis. (Mojarad, et al., 2017)
Frequently Asked Questions
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What are the common obesity susceptibility loci?
Mutations in genes in the leptin-melanocortin signaling pathway, central neuron signaling pathway, and insulin signaling pathway are important factors that increase the risk of obesity, such as MC4R, the leptin gene (LEP), and other genes. There are also some genes that do not have clear metabolic pathways but are related to energy metabolism and thermogenesis, such as FTO, UCP, the ankyrin-repeat kinase domain containing 1 gene (ANKK1), and so on.
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What is the functional profile of CEP19?
The protein encoded by the CEP19 gene is localized to centrosomes and primary cilia and co-localizes with markers on the mother centromere. The gene is located in the region of human chromosome 3 associated with morbid obesity. Homozygous knockout of the gene in mice results in morbid obesity, hyperphagia, glucose intolerance, and insulin resistance. Specifically, the CEP19 gene has a pseudogene on human chromosome 2.
Based on professional gene sequencing, Protheragen provides high-quality CEP19 gene testing to help clients explore the mechanisms of genetic obesity. Whether you want to customize a gene-edited animal model or a single gene test, please feel free to contact us for the most convenient solution.
Reference
- Mojarad, B.A.; et al. CEP19 cooperates with FOP and CEP350 to drive early steps in the ciliogenesis programme. Open Biology. 2017, 7(6): 170114. (CC BY 4.0)
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.