Overview

Metabolic syndrome (MetS) is a growing public health problem worldwide. Dietary habits often influence the risk of obesity, and nutrient intake behaviors and energy transformations vary among populations with different genetic backgrounds. Single-nucleotide polymorphisms (SNPs) are the main form of variation in the human genome sequence that regulates gene expression. Protheragen focuses on providing Obesity Causation Analysis solutions to meet the different needs of our clients in Genetically Induced Obesity Research.

The LEP gene is the earliest obesity susceptibility gene discovered. It is located in the leptin-melanocortin signaling pathway and encodes leptin, which is expressed only in mature adipocytes. The LEP gene regulates body weight and body fat through the feeding center of the hypothalamus, which helps to reduce the risk of obesity.

Associations of LEP Gene Variants with Obesity

Genotyping of SNPs

Protheragen provides professional genotype distribution and allele frequency testing services for LEP polymorphisms. Our company provides genomic DNA extraction services by the precipitation method. Our researchers use polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) to genotype polymorphisms in the LEP gene. We use agarose gel electrophoresis and polyacrylamide gel electrophoresis for the detection and separation of PCR products and restriction digest products.

Functional Nonsynonymous SNPs (nsSNPs) Analysis

Protheragen provides prediction services and structural stability analysis services for harmful nsSNPs in the LEP gene. Relying on advanced prediction tools and comprehensive databases, our company provides conservation, stability, and structural analysis services for high-risk nsSNPs. Moreover, we provide different interaction analysis services between different amino acids in wild and mutant proteins.

Workflow

Based on advanced technology and reliable prediction tools, we provide an accurate LEP gene test service. Our researchers provide high-quality gene test services, including genotype distribution analysis and an nsSNPs prediction service, which helps clients to comprehensively interpret the mechanism of the LEP gene and the occurrence of obesity.

Applications

• The LEP gene test can be used to identify and validate prognostic indicators in patients with acute myeloid leukemia.
• The LEP gene mutations play an important role in causing obesity in humans and can be used as drug targets for the development of anti-obesity therapies.
• The LEP gene test can be used to reveal associations between genetic variants and energy intake.

Advantages of Us

• Our company has advanced prediction tools and modeling methods to effectively help clients resolve mutation sites and interactions.
• Our highly trained bioinformatics team provides data screening, organization, and visualization services.
• Our company has a strict analytical process to provide clients with the most satisfactory analytical services in the shortest possible time.

Comprehensive Obesity Research Program

Publication Data

Frequently Asked Questions

1. What is the LEP gene?

   The LEP gene, also known as the obesity gene (ob), is located at 7q31.3. The LEP gene encodes a protein that is released into the circulation by white fat cells. Circulating leptin binds to leptin receptors in the brain and activates signaling pathways that effectively suppress appetite and accelerate energy expenditure. In addition, leptin has a variety of endocrine functions and is involved in the regulation of immune response, inflammation, neovascularization, bone formation, wound healing, etc. Mutations in the LEP gene or its regulatory regions lead to morbid obesity in the form of severe obesity and hypogonadotropic hypogonadism.

2. What are the diseases associated with the LEP gene?

   Diseases associated with the LEP gene include morbid obesity, overnutrition, eating disorders, bulimia nervosa, severe pre-eclampsia, glucose intolerance, anorexia nervosa, hyperinsulinism, acquired generalized lipodystrophy, gestational diabetes, nutritional deficiency disease, hypogonadism, protein-energy malnutrition, fatty liver disease, acanthosis nigricans, prediabetes syndrome, etc.

Protheragen continues to improve the LEP gene testing process and is committed to providing the most convenient service to our clients. Our goal is to be your best assistant in the field of obesity research. No matter what you need, feel free to contact us to help you!

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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