Overview

Obesity is a complex, multifactorial health problem involving the interaction of multiple factors such as genetics, environment, and lifestyle. Among them, Genetic Factors play an important role in the occurrence and development of obesity. Relying on a professional analysis team, Protheragen provides high-quality Obesity Causation Analysis. Moreover, our company offers a professional Monogenic Obesity Gene Test Service to analyze the effects of individual genes on obesity accurately. Variations in the brain-derived neurotrophic factor (BDNF) gene, one of the key nerve growth factors, have been significantly associated with weight management, energy balance, appetite regulation, and exercise capacity in individuals.

Join Us in Deciphering the Correlation Between the BDNF Gene and Obesity

BDNF gene significantly affects feeding behavior, energy expenditure, glucolipid metabolism, and body weight changes in animals and humans. It has direct or indirect regulatory effects on energy metabolic activities. There is a strong need for a comprehensive assessment of the impact of metabolic diseases such as obesity.

Genetic Engineering Animals

• Our company has a wealth of animal models and reliable modeling techniques to help clients construct unique animal models.
• Our company provides high-quality recombinant adeno-associated virus (AAV) vector construction and stereotaxic surgery by skilled researchers. All animals are carefully fed and monitored until fully recovered.
• Depending on the client's research needs, we provide various vector construction services such as AAV-autoBDNF and destabilized yellow fluorescent protein control (AAV-dsYFP).

Professional Monitoring System

• Based on an advanced monitoring system, we provide reliable food intake and body weight tests, body temperature, energy expenditure, open field tests, and elevated plus maze services.
• Our lab offers professional glucose tolerance tests (GTT), insulin tolerance tests (ITT), and echo magnetic resonance imaging (echoMRI) scanning. These tests better interpret the effects of the BDNF gene on obesity.
• For post-execution animals, our company provides a variety of services to comprehensively assess the BDNF gene, as follows:
  • Serum harvest and analysis
  • Western blotting

  • Biomarker analysis
  • Light microscope

  • Adipose tissue analysis
  • Liver histology analysis
  • Immunohistochemistry
  • Hepatic triglyceride measurement

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR)
  • BDNF enzyme-linked immunosorbent assay (ELISA)
• Through advanced gene sequencing technology, our researchers provide site-specific analysis of the BDNF gene. Our researchers provide mutation and phenotypic analyses to help clients gain insight into the mechanisms by which the gene affects obesity.

Workflow

Our company provides one-stop BDNF gene test services from protocol design to data report delivery. According to the client's needs, we provide customized animal models and AAV vector construction solutions. Subsequently, our professional team provides stereotactic surgery and comprehensive post-operative testing services. Through a series of specialized tests and assessments, the physiological and psychological effects of the BDNF gene on the animal are explored in depth. Moreover, we provide comprehensive tissue sample analysis services in conjunction with molecular biology techniques to reveal the mechanism of gene action. We have a highly efficient data analysis team to provide clients with data screening and visualization services.

Applications

• BDNF gene test can be used to analyze the molecular mechanisms and signaling pathways involved in the regulation of energy metabolism and obesity.
• BDNF gene test can be used to study the mechanisms of many diseases such as Alzheimer's disease, gliomas, epilepsy, and depression.
• BDNF gene test can be used to regulate and analyze glycolipid metabolism.

Advantages of Us

• Our company offers not only typical neurological BDNF gene testing but also non-neurological (adipose tissue and skeletal muscle) BDNF gene testing.
• According to the different research needs of clients, our company supports personalized animal model construction and genetic analysis route analysis.
• Our researchers have proven experience in animal husbandry and metrics evaluation to provide clients with the most accurate results.

Explore the Obesity Story with Us

Publication Data

Frequently Asked Questions

1. How does the BDNF gene affect fat metabolism in the body?

   BDNF gene is involved in the multifaceted regulation of energy metabolism through interactions with tropomyosin receptor kinase B (TrkB) and p75 neurotrophic factor receptor (p75NTR), including participation in central nervous system regulation and energy uptake and expenditure in peripheral metabolic organs.

2. How to detect BDNF expression?

   Our company uses western blotting to detect BDNF protein expression. Our researchers obtain protein samples by lysing adipose tissue and centrifuging it. We use the bicinchoninic acid (BCA) assay to determine the protein concentration separately. After quantification, our researchers make electrophoretic samples. Moreover, we quantify BDNF protein expression and analyze it through a series of electrophoretic separations, transfer, containment, incubation, and membrane washing.

Relying on advanced analysis technology, Protheragen provides professional BDNF gene test services to clients. Our goal is to help clients deeply interpret the regulatory mechanisms of BNDF genes. Please feel free to contact us if you need help.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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