SH2B1 Gene Test Service
InquiryOverview
The SH2B1 gene has become a significant focus in the intricate study of obesity. Its connection to obesity is complex, as it plays a role in how the body reacts to hormonal cues that regulate hunger and fullness, thus impacting both food consumption and energy use. Variations or mutations within this gene can interfere with these essential regulatory processes, potentially leading to conditions that promote excessive weight gain. By recognizing individuals who possess particular genetic characteristics linked to the SH2B1 gene, healthcare professionals can tailor dietary plans, exercise regimens, and treatment approaches for more effective weight management and reduction of related health risks.
Discover your genetic potential with our SH2B1 Gene Test Service!
Protheragen is actively involved in obesity research, consistently investigating the latest scientific discoveries to guarantee that we offer the most effective and optimal solutions for our clients. Our research encompasses several key areas, such as the exploration of new treatment options through the Development of Anti-Obesity Therapeutic based on different targets. These targets encompass various options, such as Hormones and Peptides, Receptors, Enzymes and Proteins, Metabolic Regulators, and Signaling Molecules. Furthermore, we are committed to enhancing Anti-Obesity Therapies, which include the development of small-molecule drugs, gene therapy, cell therapy, antibody therapy, tissue-engineering therapy, and nanotherapy.
In addition, our preclinical studies validate the safety and effectiveness of these novel anti-obesity therapies as we aim to address the obesity issue and improve individuals' health and quality of life.
| Preclinical Studies of Anti-Obesity Therapeutics Our team of highly skilled professionals is committed to performing thorough investigations and evaluations to determine the effectiveness, safety, and potential of these therapies before their progression into clinical trials. |
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While conducting obesity-related scientific research, Protheragen also provides you with expert Obesity Causation Analysis Services, including but not limited to the obesity causation analysis of Genetically Induced Obesity, Disease-Induced Obesity, and Medication-Induced Obesity. Among these offerings, our SH2B1 gene testing service is detailed as follows.
Mutation Screening
We first collect DNA samples from individuals, polymerase chain reaction (PCR)-amplify the coding region of the SH2B1 gene using specific primers, and then analyze the PCR products using denaturing high-performance liquid chromatography (dHPLC) to detect the mutation. dHPLC has a similar or higher accuracy than sequencing. Subsequently, we resequence all samples in which the dHPLC pattern differs from the wild-type pattern to confirm the presence of the mutation.
Functional Analysis
Our high-throughput sequencing technology and bioinformatics tools allow us to systematically assess the impact of these mutations on the overall gene expression profile. The mutations are analyzed in detail to investigate whether they result in the loss or gain of cryptic splice sites and transcription factor binding sites. These changes may affect the normal function of the gene, thereby altering protein synthesis and regulation.
Genotyping
At this stage, we perform detailed genotyping of the identified variants to determine their specific types and characteristics. Commonly used methods include restriction fragment length polymorphism (RFLP) and others. These techniques are effective in identifying different alleles and provide information about the frequency of mutations, thus helping us to understand the distribution of these variants in the population.
Workflow
Applications
- For people with a family history of obesity, the test can assess their genetic susceptibility and enable targeted lifestyle interventions, such as personalized diet and exercise plans, to be taken in advance to reduce the risk of obesity occurring.
- The test is used to tailor weight loss programs for dieters, including the selection of exercise modalities and intensities that are better suited to an individual's genetic profile, as well as the development of targeted dietary regimens.
- The test plays a role in the prevention and management of obesity-related complications. Information on the SH2B1 gene status can help predict the risk of possible complications of obesity, such as cardiovascular disease and diabetes, so that interventions can be taken in advance to reduce the chance of complications.
Advantages
- We use advanced gene sequencing technology and sophisticated analytical algorithms to accurately identify variants and polymorphisms in the SH2B1 gene, reducing the likelihood of misclassification.
- We perform comprehensive testing on all key regions of the SH2B1 gene to ensure that no important loci are missed and to provide more complete information about the gene.
- Protheragen continues to introduce the latest genetic testing technology and research results to keep the testing technology advanced and scientific.
Publication Data
Technologies: Gene sequencing, Cell migration assay
Journal: Endocrinology
Published: 2014
IF: 3.8
Results: This article explores the functional characterization of SH2B1 gene variants associated with obesity. Specifically, the article analyzes the roles of the different isoforms (α and β) of SH2B1 in the regulation of energy balance and glucose homeostasis. There are four isoforms (α, β, δ, and γ) of SH2B1, which share structural similarities but differ in the length of the C-terminal end and their expression patterns. It was shown that the β and γ isoforms were widely expressed, while the α and δ isoforms were mainly expressed in brain regions. The article mentions that although little is known about the physiological relevance of the different SH2B1 isoforms, specific restoration of the β isoform rescues the obesity phenotype in a murine model deficient in SH2B1. In addition, the article describes several obesity-related SH2B1 variants that affect the cellular function of SH2B1 and are associated with severe early-onset obesity and insulin resistance.
Fig.1 Discovery of new variants in the SH2B1 gene. (Pearce, et al.,2014)
Frequently Asked Questions
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What does it mean if the test results show a mutation in the SH2B1 gene?
Variants may make individuals more susceptible to weight gain and obesity, but this is not absolute and only indicates an increased risk. At the same time, it may suggest that there is a higher likelihood of developing metabolic diseases, which are closely related to weight and metabolic regulation. In controlling body weight and maintaining good health, dietary and exercise interventions may need to be adjusted differently from the norm to achieve better results.
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Do SH2B1 gene variants always lead to obesity or related diseases?
SH2B1 gene variants do not necessarily cause obesity or related diseases. Although a gene variant may increase susceptibility to obesity and related diseases, it is not the only factor in determining whether or not a disease develops. Lifestyle, including diet, exercise, and stress management, has a significant impact on weight and health status.
Protheragen offers advanced and professional SH2B1 gene testing services. Our testing services rely on cutting-edge technology and precise experimental procedures to provide comprehensive and in-depth analysis of the SH2B1 gene. Both subtle changes in the gene sequence and potential mutation sites can be accurately identified and evaluated. Our dedicated team is here to assist you, please feel free to contact us!
Reference
- Pearce, L.R.; et al. Functional characterization of obesity-associated variants involving the α and β isoforms of human SH2B1. Endocrinology. 2014, 155(9): 3219-3226. (CC BY 3.0)
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.