POMC Gene Test Service
InquiryOverview
The POMC gene holds a pivotal role in the comprehension and resolution of weight-related issues. It is integral to the regulation of energy homeostasis and appetite control within the body. Mutations or variations in the POMC gene can disrupt normal physiological functions, potentially leading to metabolic dysregulation that heightens susceptibility to weight gain and obesity. Investigations into the POMC gene have unveiled new pathways for elucidating the intricate mechanisms underlying this prevalent health concern. This research aids in identifying individuals with a genetic predisposition to obesity and offers critical insights for formulating targeted therapeutic strategies and preventive interventions.
Unveil Genetic Secrets with Our POMC Gene Test
Protheragen is dedicated to advancing research focused on obesity, particularly in the development of innovative treatment solutions for this challenge. Our investigations cover essential domains such as Identifying New Targets for Anti-Obesity Treatments, including Hormones and Peptides, Receptors, Enzymes and Proteins, Metabolic Regulators, and Signaling Molecules. Furthermore, we are focused on progressing the development of Anti-Obesity Therapies like small-molecule drugs, gene therapy, cell therapy, antibody therapy, tissue engineering therapy, and nanotherapy.
We additionally perform Preclinical Studies on anti-obesity therapeutics to confirm their safety and efficacy. These studies are crucial in the drug development process, as they allow us to evaluate the pharmacological properties of potential treatments before they are tested in human clinical trials.
Keeping this in consideration, we provide our clients with a diverse array of genetic testing services, which encompass Monogenic Obesity Gene Tests as well as Obesity Gene Panel Tests among other options. The details of our POMC gene test service are as follows.
Pre-sequencing Preparation
First, our experts extract genomic DNA from the collected samples. Commonly used methods include phenol/chloroform extraction or commercially available DNA extraction kits. Meanwhile, to verify the purity and concentration of the extracted DNA, we use a spectrophotometer for measurement. Through these steps, we obtain a sufficient amount and high-quality of genomic DNA to provide reliable data support for subsequent experiments. Subsequently, we perform polymerase chain reaction (PCR) amplification of specific regions of the POMC gene using primers specifically designed for the POMC gene to ensure effective amplification of the target fragments.
Sequencing
Our amplified PCR products are sequenced by Sanger sequencing to confirm the specific sequence variation of the POMC gene. In addition, we also use restriction fragment length polymorphism (RFLP) analysis to cut the PCR product with specific restriction enzymes. After digestion, we use agarose gel electrophoresis to separate the individual fragments and determine whether there are different types of genetic variants in the samples based on their sizes and numbers.
Analysis and Interpretation of Results
Our experts make detailed judgments about the status of the POMC genes in the samples based on the sequencing data, including the presence of deletions, mutations, or other types of genetic variants. By comparing the obtained sequences with the reference genome, we identify specific changes and assess the possible biological significance of these changes. For example, certain specific mutations may affect the function of the protein encoded by the POMC gene, with potential implications in the regulation of appetite and energy metabolism.
Workflow
The workflow of our POMC gene test service is as follows.
Applications of Our POMC Gene Test Service
- It helps identify genetic variations in the POMC gene that may contribute to the development of obesity and related metabolic disorders.
- It is used to provide valuable information for families with a history of obesity or related conditions, assisting in making informed decisions about reproduction and potential risks.
- It facilitates studies on the underlying mechanisms of obesity and the development of new therapeutic approaches.
Advantages
- Our service is capable of precisely detecting variations and mutations in the POMC gene, minimizing the chances of false positives or negatives.
- We target the specific regions of the POMC gene related to obesity and related disorders, providing focused and relevant information.
- Our technology identifies even minor genetic alterations that might have significant implications for an individual's health.
Publication Data
Technologies: Sanger sequencing
Journal: Journal of Veterinary Internal Medicine
Published: 2017
IF: 2.19
Results: This paper focuses on the relationship between a 14 base pair deletion in the POMC gene and obesity in Labradors, and whether this gene deletion is associated with the risk of diabetes mellitus (DM). Primers were designed in the study to amplify a fragment containing 560 base pairs of exon 3 of the canine POMC gene, and PCR reactions and subsequent analyses were performed. It was found that although POMC gene deletion was associated with obesity and appetite, there was no evidence of an association between this gene deletion and the risk of diabetes in Labradors. Overall, the study suggests that the risk of diabetes in Labradors is not driven by obesity.
Fig.1 Sanger sequencing results of dogs exhibiting homozygous, heterozygous, and wild-type genotypes concerning the 14-base-pair deletion in the POMC gene. (Davison, et al., 2017)
Frequently Asked Questions
-
Who is a candidate for the POMC gene test?
Individuals with a family history of obesity, unexplained obesity, or metabolic disorders, and an interest in their genetic risk for obesity may be candidates for this test.
-
Is this test a one-time event, or does it need to be repeated periodically?
Typically, for most people, the POMC gene test is considered a one-time test, as it is primarily designed to identify an individual's genetic risk factors. However, in some specific cases, reassessment may be recommended. For example, if a new case of obesity or related metabolic disease appears in the family, this may suggest the presence of an undetected genetic variant in the family, prompting a re-examination of the results already available.
Protheragen offers detailed POMC testing services designed to provide comprehensive insights into genetic factors associated with obesity and related metabolic disorders. Our cutting-edge laboratory employs sophisticated technologies and techniques to guarantee precise and dependable results. If you have any questions, concerns, or require further information regarding our services, please feel free to contact us!
Reference
- Davison, L.J.; et al. The canine POMC gene, obesity in Labrador retrievers and susceptibility to diabetes mellitus. Journal of Veterinary Internal Medicine. 2017, 31(2): 343-348. (CC BY 4.0)
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.