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Uric acid assay kit (Colorimetric method - 96T)
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Cat. No.:
OB0125WXX-019
Synonyms:
Uric acid assay kit; Uric acid
Size:
96T
Specification
Description:
Uric acid assay kit is a metabolic test kit for detecting uric acid by the colorimetric method in the range of 1.30-80 mg/L.
Background:
Uric acid is a purine metabolite that is degraded to allantoin by uricase in most mammals. It is the end product of purine metabolism in humans due to the lack of the uric acid oxidase gene, and therefore, human blood levels of uric acid are higher than in most mammals. Uric acid is an important physiological plasma antioxidant that effectively protects biological targets from oxidation by hydroxyl radicals, hypochlorous acid, and peroxynitrite.
Assay Type:
Quantitative assay
Assay Principle:
Reduction of phosphotungstic acid by uric acid in protein-free filtrates produces tungsten blue, allantoin, and carbon dioxide, with the shade of blue being proportional to the concentration of uric acid. Uric acid content is calculated by measuring the OD at 690 nm.
Assay Target:
Uric acid
Target Species:
Universal
Research Area:
Kidney biomarkers; Oxidative stress; ATP and related research; Obesity
Sample Type:
Serum; Plasma; Urine
Kit Components:
Uric acid standard (1 g/L); Protein precipitator; Alkali reagent; Phosphotungstic acid reagent; Microplate; Plate sealer
Detection Range:
1.30-80 mg/L
Sensitivity:
1.30 mg/L
Precision (Intra-assay):
2% (Average)
Precision (Inter-assay):
4% (Average)
Reaction Time:
35 min
Recovery:
0.96
Applications:
Uric acid assay kit is used to measure the uric acid (UA) content in plasma, serum, and urine samples.
Storage:
Store at 2-8°C.