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Uric acid assay kit (Fluorimetric method)
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Cat. No.:
OB0125WXX-018
Synonyms:
Uric acid assay kit; Uric acid
Size:
96T
Specification
Description:
Uric acid assay kit is a metabolic test kit for detecting uric acid by the fluorometric method in the range of 0.03-15μmol/L.
Background:
Uric acid is a purine metabolite that is degraded to allantoin by uricase in most mammals. It is the end product of purine metabolism in humans due to the lack of the uric acid oxidase gene, and therefore, human blood levels of uric acid are higher than in most mammals. Uric acid is an important physiological plasma antioxidant that effectively protects biological targets from oxidation by hydroxyl radicals, hypochlorous acid, and peroxynitrite.
Assay Type:
Quantitative assay
Assay Principle:
The enzyme uricase catalyzes the decomposition of uric acid into allantoin, carbon dioxide, and H2O2. In the presence of peroxidase, H2O2 oxidizes the non-fluorescent probe into a fluorescent substance. By measuring the fluorescence value of the system, the corresponding uric acid content is calculated.
Assay Target:
Uric acid
Target Species:
Universal
Research Area:
Kidney biomarkers; Oxidative stress; ATP and related research; Obesity
Sample Type:
Serum; Plasma; Urine
Kit Components:
Buffer solution; Probe solution; Enzyme reagent 1; Enzyme reagent 2; Uric acid standard (20 μmol/L); Black microplate; Plate sealer
Detection Range:
0.03-15 μmol/L
Sensitivity:
0.03 μmol/L
Precision (Intra-assay):
1.5% (Average)
Precision (Inter-assay):
7.2% (Average)
Reaction Time:
40 min
Recovery:
1.01
Applications:
Uric acid assay kit is used to measure the uric acid (UA) content in urine, plasma, serum, and animal tissue samples.
Storage:
Store at 2-8°C.