Non-esterified free fatty acids (NEFAs) assay kit (Colorimetric, 0.15-1.5 mmol/L)

Cat. No.: OB0525WXX-412

Assay Type: Quantitative assay

Target Species: Universal

Assay Target: NEFA

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Non-esterified free fatty acids (NEFAs) assay kit (Colorimetric, 0.15-1.5 mmol/L)

INQUIRY Datasheet MSDS

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Product Overview

Description: Non-esterified free fatty acids (NEFAs) assay kit (Colorimetric, 0.15-1.5 mmol/L) is a metabolic assay kit for the rapid and sensitive detection of NEFA in the range of 0.15-1.5 mmol/L.

Assay Principle: Under weak acidic conditions, NEFA reacts with nantokite to form copper soap, which has a specific absorption peak at 715 nm. The content of NEFA can be calculated indirectly by measuring the OD value at 715 nm.

Background: Free fatty acids, also known as non-esterified fatty acids (NEFA), are derived from the diet or metabolism of adipose tissue. In adipose tissue, hormone-sensitive lipase (HSL) breaks down triglycerides to produce glycerol and fatty acids. When circulating in the body, free fatty acids are bound to plasma albumin and serve as a source of energy that is readily absorbed by muscle, brain, and other tissues and organs. Non-esterified free fatty acid (NEFA) is not only a product of fat hydrolysis, but also a substrate for fat synthesis. The concentration of NEFA has been linked to lipid metabolism, glucose metabolism, and endocrine function.

Synonyms: FFA; Free fatty acids; Unesterified fatty acids; Unbound fatty acids

Applications: Non-esterified free fatty acids (NEFAs) assay kit (Colorimetric, 0.15-1.5 mmol/L) is used to measure the NEFAs content in animal tissue samples.

Research Area: Obesity research

Specification

Sample Type: Tissue; Cells

Kit Components: Extracting solution; Palmitic acid standard (10 mmol/L); Control solution; Reaction solution; Microplate; Plate sealer

Detection Method: Colorimetric method

Detection Range: 0.15-1.5 mmol/L

Sensitivity: 0.15 mmol/L

Precision (Intra-assay): 0.051

Precision (Inter-assay): 0.033

Reaction Time: 30 min

Recovery: 1.01

Storage: Store at 2-8°C.

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