Oxalate (oxalic acid) assay kit (Colorimetric)

Cat. No.: OB0525WXX-409

Assay Type: Quantitative assay

Target Species: Universal

Assay Target: Oxalate (oxalic acid)

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Oxalate (oxalic acid) assay kit (Colorimetric)

INQUIRY Datasheet MSDS

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Product Overview

Description: Oxalate (oxalic acid) assay kit (Colorimetric) is a metabolic assay kit for the rapid and sensitive detection of oxalate (oxalic acid) in the range of 0.02-1 mmol/L.

Assay Principle: Oxalate oxidase catalyzes the oxidation of oxalate to produce hydrogen peroxide and carbon dioxide. In the presence of POD, hydrogen peroxide reacts with the chromophore to produce a colored product. There is a specific absorption peak at 550 nm, and the shade of color is proportional to the amount of oxalate.

Background: Oxalic acid or oxalate is a metabolic breakdown product of the glyoxylate cycle in eukaryotes. Oxalate concentrations in human urine and other mammals can be used to assess renal function. Excessive levels of oxalate may lead to the formation of kidney stones.

Synonyms: Oxalic acid; Ethanedioate

Applications: Oxalate (oxalic acid) assay kit (Colorimetric) is used to measure oxalate content in animal urine, serum, plasma, and plant tissue samples.

Research Area: Obesity research; Food safety and analysis

Specification

Sample Type: Urine; Serum; Plasma; Plant tissue

Kit Components: Buffer solution; Working solution; Chromogenic agent A; Chromogenic agent B; Enzyme reagent A; Enzyme reagent B; Regulator; Clarificant; Standard (1 mmol/L); Microplate; Plate sealer

Detection Method: Colorimetric method

Detection Range: 0.02-1 mmol/L

Sensitivity: 0.02 mmol/L

Precision (Intra-assay): 0.047

Precision (Inter-assay): 0.03

Reaction Time: 35 min

Recovery: 0.95

Storage: Store at 2-8°C.

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