L-Lactic acid/lactate assay kit (Colorimetric)

Cat. No.: OB0525WXX-421

Assay Type: Quantitative assay

Target Species: Universal

Assay Target: L-Lactic acid/lactate

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L-Lactic acid/lactate assay kit (Colorimetric)

INQUIRY Datasheet MSDS

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Product Overview

Description: L-Lactic acid/lactate assay kit (Colorimetric) is a metabolic assay kit for the rapid and sensitive detection of L-Lactic acid/lactate in the range of 0.12-7.0 mmol/L.

Assay Principle: LDH catalyzes the reaction of lactic acid and NAD⁺ to form pyruvic acid and NADH, respectively, using NAD⁺ as the H⁺ receptor, during which NBT is reduced to a purple compound. The concentration of lactate can be calculated by measuring the OD at 530 nm.

Background: Lactic acid is an intermediate product of glucose metabolism in the body and is produced mainly by red blood cells, rhabdomyosin, and brain tissue. The concentration of lactic acid in the blood depends mainly on the rate of synthesis and metabolism in the liver and kidneys. The bidirectional conversion of lactate and pyruvate is regulated by lactate dehydrogenase (LDH).

Synonyms: L-Lactate; L-Lactic acid; (S)-2-hydroxypropanoic acid

Applications: L-Lactic acid/lactate assay kit (Colorimetric) is used to measure L-lactic acid (LA) content in serum (plasma), tissue, cells, and culture supernatant samples.

Research Area: Food safety and analysis; Obesity research; Glycolysis and carbohydrates

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Tissue; Cells

Kit Components: Buffer solution; Enzyme stock solution; Chromogenic agent; Stop solution; Standard (10 mmol/L); Microplate; Plate sealer

Detection Method: Colorimetric method

Detection Range: 0.12-7.0 mmol/L

Sensitivity: 0.10 mmol/L

Precision (Intra-assay): 0.035

Precision (Inter-assay): 0.014

Reaction Time: 30 min

Recovery: 1.05

Storage: Store at 2-8°C.

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