Succinate assay kit

Cat. No.: OB0525WXX-403

Assay Type: Quantitative assay

Target Species: Universal

Assay Target: Succinate

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Product Overview

Description: Succinate assay kit is a metabolic assay kit for the rapid and sensitive detection of succinate.

Assay Principle: Tetrazolium salt MTT is reduced to reduced MTT in the NADH-coupled enzymatic reaction and shows an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

Background: Succinic acid is found in all animal tissues. It is an intermediate in the citric acid cycle and plays an important role in intracellular energy production. Due to its low toxicity, succinic acid is widely used as a flavoring agent in the food, beverage, and pharmaceutical industries.

Synonyms: Succinic acid; Butanedioate

Applications: Succinate assay kit is used to measure succinate (succinic acid) in food, beverages, agricultural products, and other biological samples.

Research Area: TCA cycle; Obesity research; Food safety and analysis

Specification

Sample Type: Cells; Tissue

Kit Components: Assay buffer; Enzyme Mix; Co substrate; PEP; Standard

Detection Method: Colorimetric method (OD 570 nm); Fluorimetric method (FL 530/585 nm)

Detection Limit: 10 µM (OD); 2 µM (FL)

Reaction Time: 30 min

Storage: Store at 2-8°C.

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