Products
Online Inquiry
Lactase activity assay kit
Inquiry
Cat. No.:
OB0125WXX-008
Synonyms:
Lactase; Activity assay kit; Lactase assay kit; EC 3.2.1.108
Size:
96T
Specification
Description:
Lactase activity assay kit is an enzyme activity test kit for the rapid and sensitive detection of lactase in the range of 12.5-2000 U/mL.
Background:
Lactase is a glycoprotein with two active sites that catalyze the hydrolysis of various β-glycosidic bonds. It catalyzes the breakdown of lactose into glucose and galactose. Lactase plays a crucial role in neonatal nutrition in humans and other mammals because this enzyme is the only small intestinal brush border hydrolase responsible for the digestion of lactose. Low lactase levels and inability to digest lactose may lead to lactose intolerance.
Assay Type:
Activity assay
Assay Principle:
Lactase breaks down lactose to produce glucose. In the presence of the enzyme, glucose produces hydrogen peroxide. In the presence of a chromogenic oxygen receptor, peroxidase catalyzes the production of a colored substance from hydrogen peroxide. Lactase activity can be calculated by measuring the OD at 505 nm.
Assay Target:
Lactase
Target Species:
Universal
Research Area:
Glycolysis and carbohydrates; Enzyme; Obesity
Sample Type:
Tissue
Kit Components:
Substrate; Buffer solution; Stop solution; Phenol solution; Enzyme solution; Glucose standard solution; Microplate; Plate sealer
Detection Range:
12.5-2000 U/mL
Sensitivity:
3.94 U/mL
Precision (Intra-assay):
4.5% (Average)
Precision (Inter-assay):
8.5% (Average)
Reaction Time:
60 min
Recovery:
1.02
Applications:
Lactase activity assay kit is used to measure lactase activity in animal tissue samples, which has important applications in glycolysis, obesity, and other studies.
Storage:
Store at 2-8°C.