Products
Online Inquiry
Acetylcholinesterase (AchE) activity assay kit
Inquiry
Cat. No.:
OB0125WXX-001
Synonyms:
AchE; AchE assay kit; Acetylcholinesterase (AchE) activity; Acetylcholinesterase; EC 3.1.1.7
Size:
96T
Specification
Description:
Acetylcholinesterase (AchE) activity assay kit is an enzyme activity test kit for the rapid and sensitive detection of AchE in the range of 1.225-490 U/mL.
Background:
AchE is a serine hydrolase with monomeric, dimeric, and tetrameric structures containing one, two, or four catalytic subunits found primarily in the brain at cholinergic synapses and neuromuscular junctions. Its primary biological role is the rapid hydrolysis of the neurotransmitter acetylcholine to acetate and choline to terminate impulsive transmission at cholinergic synapses.
Assay Type:
Activity assay
Assay Principle:
AchE catalyzes the hydrolysis of acetylcholine to form choline, which reacts with p-nitrobenzoic acid to form 5-mercapto-nitrobenzoic acid (TNB). TNB has an absorption peak at 412 nm. The activity of AchE is calculated by measuring the rate of increase in absorbance at 412 nm.
Assay Target:
AchE
Target Species:
Universal
Research Area:
Lipid metabolism; Enzymes; Obesity
Sample Type:
Serum; Plasma; Cells; Culture supernatent; Tissue
Kit Components:
Lysis buffer; Buffer solution; Chromogenic agent; Substrate; Microplate; Plate Sealer
Detection Range:
1.225-490 U/mL
Sensitivity:
1.225 U/mL
Precision (Intra-assay):
4.7% (Average)
Precision (Inter-assay):
9.3% (Average)
Reaction Time:
20 min
Recovery:
1.04
Applications:
Acetylcholinesterase (AchE) activity assay kit is used to measure AchE content in serum, plasma, and animal tissue samples.
Storage:
Store at 2-8°C.