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Chicken adiponectin (ADPN) CLIA kit
Cat. No.:
OB0125WXX-031
Assay Type:
Double-antibody sandwich CLIA
Target Species:
Chicken
Assay Target:
ADPN
Size:
Product Overview
Description:
Chicken adiponectin (ADPN) CLIA kit is a CLIA kit assay for the detection of chicken ADPN levels. The range of the assay is 10.97-8000 pg/mL.
Assay Principle:
The microtiter plates in this kit are pre-coated with an antibody specific for ADPN. Standards or samples are then added to the corresponding microtiter plate wells along with a biotin-conjugated antibody specific for ADPN. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter well and incubated. A mixture of substrates A and B is then added to produce glow emission kinetics. After the microtiter plate is developed, the intensity of the glow is proportional to the level of ADPN in the sample or standard.
Background:
Adiponectin is involved in important adipokines that control fat metabolism and insulin sensitivity. It has multiple functions, including antagonizing TNF-α by negatively regulating its expression in different tissues such as liver and macrophages and counteracting its effects; stimulating AMPK phosphorylation and activation in liver and skeletal muscle to enhance glucose utilization and fatty acid burning, and inhibiting endothelial NF-kappa-B signaling through a cAMP-dependent pathway. It is found that it may play a role in cell growth, angiogenesis, and tissue remodeling.
Synonyms:
ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin
Applications:
Chicken adiponectin (ADPN) CLIA kit is used to detect chicken ADPN levels in serum, plasma, and other biological fluid samples by chemiluminescent immunoassay.
Research Area:
Hormone research; Lipid metabolism; Obesity
Specification
Sample Type:
Serum; Plasma; Other biological fluids
Detection Range:
10.97-8,000 pg/mL
Sensitivity:
4.73 pg/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.
