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Human C reactive protein (CRP) CLIA kit
Cat. No.:
OB0125WXX-032
Assay Type:
Double-antibody sandwich CLIA
Target Species:
Human
Assay Target:
CRP
Size:
Product Overview
Description:
Human C reactive protein (CRP) CLIA kit is a CLIA kit assay for the detection of CRP level. The range of the assay is 13.7-10000 pg/mL.
Assay Principle:
The microtiter plate included in this kit is pre-coated with an antibody specific for CRP. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody specific for CRP. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter well and incubated. A mixture of substrates A and B is then added to produce glow emission kinetics. After development, the intensity of the emitted light is proportional to the level of CRP in the sample or standard.
Background:
CRP has a variety of functions related to host defense, including the ability to interact with DNA and histones and to remove nuclear material released by damaged circulating cells, and the promotion of agglutination, phagocytosis, bacterial cysts, and complement fixation through calcium-dependent binding to phosphocholine.
Synonyms:
PTX1
Applications:
Human C reactive protein (CRP) CLIA kit is used to detect CRP levels in serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernatants, and other biological fluid samples by chemiluminescent immunoassay.
Research Area:
Inflammation; Obesity
Specification
Sample Type:
Serum; Plasma; Tissue homogenates; Cell lysates; Urine; Cerebrospinal fluid; Cell culture supernates; Other biological fluids
Detection Range:
13.7-10000 pg/mL
Sensitivity:
5.1 pg/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.
