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Human adiponectin (ADP) CLIA kit
Cat. No.:
OB0125WXX-029
Assay Type:
Double-antibody sandwich CLIA
Target Species:
Human
Assay Target:
ADP
Size:
Product Overview
Description:
Human adiponectin (ADP) CLIA kit is a CLIA kit assay for the detection of ADP levels. The range of the assay is 13.7-10000 pg/mL.
Assay Principle:
The microtiter plates in this kit are pre-coated with an antibody specific for ADP. Standards or samples are then added to the appropriate microtiter plate wells, followed by the biotin-conjugated antibody specific for ADP. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter well and incubated. A mixture of substrates A and B is then added to produce glow light emission kinetics. After the microtiter plate is developed, the intensity of the glow is proportional to the level of ADP in the sample or standard.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Synonyms:
ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin
Applications:
Human adiponectin (ADP) CLIA kit is used to detect ADP levels in serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluid samples by chemiluminescent immunoassay.
Research Area:
Hormone research; Lipid metabolism; Obesity
Specification
Sample Type:
Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids
Detection Range:
13.7-10000 pg/mL
Sensitivity:
5.1 pg/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.
