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Choline acetyltransferase (ChAT) activity assay kit
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Cat. No.:
OB0125WXX-010
Synonyms:
ChAT assay kit; Choline acetyltransferase (ChAT); Activity assay kit; ChAT; EC 2.3.1.6
Size:
96T
Specification
Description:
Choline acetyltransferase (ChAT) activity assay kit is an enzyme activity test kit for the rapid and high-throughput detection of ChAT in the range of 20-2000 U/mL.
Background:
ChAT is an acetylcholine biosynthetic enzyme that produces acetylcholine by catalyzing the transfer of acetyl groups from acetyl coenzyme A to choline. ChAT is synthesized in the perinuclear body of the neuron and is transported to the neuron by a slow axonal transport mechanism. It is the most specific indicator of the functional status of cholinergic neurons of the central and peripheral nervous system.
Assay Type:
Activity assay
Assay Principle:
Catalyzed by choline acetyltransferase (ChAT), acetyl-CoA reacts with choline to form coenzyme A (CoA), which binds to 4,4-dithiopyridine. The activity of ChAT can be calculated indirectly by measuring the OD at 324 nm.
Assay Target:
ChAT
Target Species:
Universal
Research Area:
Enzyme; Obesity
Sample Type:
Tissue
Kit Components:
Buffer solution; Inhibitor; Substrate A; Substrate B; Accelerant A; Accelerant B; Chromogenic agent; UV microplate; Plate sealer
Detection Range:
1.21-40 U/g
Sensitivity:
1.21 U/g
Precision (Intra-assay):
5.1% (Average)
Precision (Inter-assay):
9.4% (Average)
Reaction Time:
65 min
Applications:
Choline acetyltransferase (ChAT) activity assay kit is used for the quantitative determination of ChAT activity in animal tissue samples, which has important applications in obesity research.
Storage:
Store at -20°C (reagents) and 2-8°C (others).