Online Inquiry

Canine interleukin 6 (IL-6) ELISA kit (15.6-1000 pg/mL)

Cat. No.: OB0125WXX-042

Assay Type: Quantitative sandwich ELISA

Target Species: Canine

Assay Target: IL-6

Size:

Canine interleukin 6 (IL-6) ELISA kit (15.6-1000 pg/mL)

INQUIRY Datasheet MSDS

Product Overview

Description: Canine interleukin 6 (IL-6) ELISA kit (15.6-1000 pg/mL) is a ready-to-use microwell, strip plate ELISA kit for analyzing the presence of canine IL-6 in the range of 15.6-1000 pg/mL.

Assay Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL-6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL-6). Next, avidin conjugated to HRP is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 (IL-6), biotin-conjugated antibody, and enzyme-conjugated avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 6 (IL-6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Background: IL-6 is a cytokine with multiple biological functions. It is a potent inducer of the acute phase response, acting on T cells, B cells, hematopoietic progenitors, hepatocytes, and cells of the central nervous system. It plays an important role in the eventual differentiation of B cells into Ig-secreting cells. It also acts as an actin, which is excreted into the bloodstream after muscle contraction and can promote lipolysis and improve insulin resistance.

Synonyms: HGF; HSF; BSF2; IL6; IFNB2; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2

Applications: Canine interleukin 6 (IL-6) ELISA kit (15.6-1000 pg/mL) is used to detect the level of canine IL-6 in the serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluid samples.

Research Area: Inflammatory research; Cytokine research; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 15.6-1,000 pg/mL

Sensitivity: 6.2 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

Related Products

Inquiry