Human pro-opiomelanocortin (POMC) ELISA kit (78-5000 pg/mL)

Cat. No.: 0126WXX-1433

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: POMC

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Human pro-opiomelanocortin (POMC) ELISA kit (78-5000 pg/mL)

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Product Overview

Description: Human pro-opiomelanocortin (POMC) ELISA kit (78-5000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of POMC in human samples with a range of 78-5000 pg/mL and a minimum detectable dose (sensitivity) of 19.5 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a quantitative sandwich enzyme immunoassay technique. An antibody specific for POMC is pre-coated onto a microplate. Standards and samples are pipetted into the wells, where any POMC present is bound by the immobilized antibody. After removing unbound substances, a biotin-conjugated detection antibody specific for POMC is added. Following a wash step, avidin-conjugated HRP is introduced. After a final wash to remove unbound reagents, a substrate solution is added, and color develops in direct proportion to the amount of POMC bound in the initial step.

Background: POMC, a highly complex precursor polypeptide belonging to the POMC family and encoded at chromosomal location 2p23.3, serves as a central hub in the endocrine regulation of energy homeostasis and systemic metabolism. Synthesized as a secreted protein that undergoes extensive peptide hormone processing, POMC is vital for the neuropeptide signaling pathways that govern appetite regulation, glucose homeostasis, and blood pressure. Its biological significance is underscored by its role in producing ACTH to stimulate adrenal cortisol release, as well as its essential binding activities at melanocortin receptors (MC3R and MC4R) to modulate feeding behavior. Crucially, genetic defects in POMC are directly linked to Pro-opiomelanocortin Deficiency (POMCD) and a heightened susceptibility to early-onset obesity—a condition defined by the excessive accumulation of body fat beyond physiological requirements. Beyond its primary metabolic functions, POMC influences diverse processes ranging from cellular pigmentation and neuropeptide transduction to the negative regulation of tumor necrosis factor production, highlighting its status as a pivotal molecular driver in the complex pathology of metabolic disorders.

Synonyms: POC; NPP; ProOMC; ACTH; Pro-OMC; Pro opiomelanocortin; Corticotropin-lipotropin; Corticotropin; Adrenocorticotropic hormone; Proopiomelanocortin

Applications: Human pro-opiomelanocortin (POMC) ELISA kit (78-5000 pg/mL) is used to quantify POMC in serum, cell culture supernatants, and tissue homogenates of human, providing data to support research in a wide range of areas, including endocrinology, obesity, and others.

Research Area: Endocrinology; Obesity

Specification

Sample Type: Serum; Cell culture supernatants; Tissue homogenates

Detection Range: 78-5000 pg/mL

Sensitivity: 19.5 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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