AdipoUpX™ Human HSD11B1 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human HSD11B1 MSCV Particle (Overexpression) is a stable integration tool for the specific and efficient overexpression of the human HSD11B1 gene. This enzyme converts inactive cortisone to active cortisol, playing a central role in visceral obesity and glucocorticoid-mediated metabolic syndrome. The vector is optimized for hematopoietic stem cells, stem cells, etc., ensuring reliable integration for longitudinal functional studies. All products undergo comprehensive quality control (titer, sterility, and mycoplasma detection) to ensure the highest research standards.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: HSD11B1

Full Name: Hydroxysteroid 11-beta dehydrogenase 1

Gene Symbol: HDL; 11-DH; HSD11; HSD11B; HSD11L; CORTRD2; SDR26C1; 11-beta-HSD1

Gene ID: 3290

RefSeq ID-1: NP_001193670.1

RefSeq ID-2: NM_001206741.2

Summary: HSD11B1 encodes a microsomal enzyme that converts cortisol to inactive cortisone and catalyzes the reverse reaction. Dysregulation can elevate cortisol levels, contributing to central obesity, and certain variants are associated with obesity and insulin resistance in children. Mutations in HSD11B1 or H6PD cause cortisone reductase deficiency. Alternative splicing produces multiple transcript variants encoding the same protein.

AdipoUpX™ Human HSD11B1 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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