Product Overview
Description:
AdipoUpX™ Human IFT27 MSCV Particle (Overexpression) is a stable integration tool for the specific and efficient overexpression of the human IFT27 gene. IFT27 is a member of the intraflagellar transport complex, and its dysfunction leads to Bardet-Biedl syndrome. The vector is optimized for stem cells and other mammalian cell lines, ensuring reliable integration for studying ciliary-mediated obesity mechanisms. Product quality is ensured by rigorous purification and comprehensive QC screening to ensure experimental results are repeatable and safe.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
IFT27
Full Name:
Intraflagellar transport 27
Gene Symbol:
RAYL; BBS19; RABL4; FAP156; CFAP156
Summary:
IFT27 encodes a GTP-binding protein that forms a core component of the intraflagellar transport complex B. Studies of the homologous protein in Chlamydomonas suggest a role in cell cycle regulation. Alternative splicing generates multiple transcript variants.
