AdipoUpX™ Human ACLY MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ACLY MSCV Particle (Overexpression) enables efficient and stable expression of the human ACLY gene, a key enzyme linking glucose metabolism to de novo lipogenesis. ACLY activity influences fatty acid synthesis and lipid accumulation in obesity-associated metabolic states. The MSCV retroviral system allows durable gene expression in stem cells and a broad range of mammalian cell models. Product quality is ensured through comprehensive QC testing, including viral titer validation and contaminant screening.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: ACLY

Full Name: ATP citrate lyase

Gene Symbol: ACL; ATPCL; CLATP

Gene ID: 47

RefSeq ID-1: NP_001087.2

RefSeq ID-2: NM_001096.2

Summary: ACLY encodes ATP citrate lyase, a central metabolic enzyme that generates cytosolic acetyl-CoA from citrate. Acetyl-CoA produced by ACLY serves as a critical substrate for de novo lipogenesis and cholesterol biosynthesis. Through its role in lipid synthesis and energy storage, ACLY is a key regulator of metabolic flux and has been implicated in obesity and related metabolic diseases. Multiple transcript variants give rise to distinct isoforms.

AdipoUpX™ Human ACLY MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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