AdipoUpX™ Human PRKAR1A MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PRKAR1A MSCV Particle (Overexpression) is a high-titer MSCV retroviral tool designed for the stable expression of the protein kinase A regulatory subunit 1A. It is an essential resource for studying PKA-mediated lipolysis and energy expenditure in adipocytes. The MSCV system ensures stable, high-level gene integration within hematopoietic stem cells, stem cells, and various mammalian cell lines. Rigorous testing for titer, sterility, and mycoplasma is performed on every batch to provide a reliable tool for studying the metabolic consequences of cAMP signaling.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: PRKAR1A

Full Name: Protein kinase cAMP-dependent type I regulatory subunit alpha

Gene Symbol: CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1; Prkar1alpha

Gene ID: 5573

RefSeq ID-1: NP_001263218.1

RefSeq ID-2: NM_001276289.2

Summary: The PRKAR1A gene encodes a regulatory subunit of the cAMP-dependent protein kinase (PKA) complex. It is a critical transducer of cAMP signaling, which regulates cellular processes ranging from nutrient metabolism to DNA replication. Mutations in this gene lead to Carney complex and are linked to various endocrine tumors.

AdipoUpX™ Human PRKAR1A MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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