AdipoUpX™ Human PNLIP MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PNLIP MSCV Particle (Overexpression) is a stable integration tool for the specific and efficient overexpression of the human PNLIP gene. Pancreatic lipase is the primary enzyme responsible for dietary fat digestion, and its inhibition is a key therapeutic strategy for weight management. By utilizing a vector optimized for stem cells and various mammalian cell types, researchers can study the regulation of lipid absorption in permanent cell models. Product quality is ensured by rigorous purification and comprehensive QC screening, ensuring data accuracy and safety.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: PNLIP

Full Name: Pancreatic lipase

Gene Symbol: PL; PTL; PNLIPD

Gene ID: 5406

RefSeq ID-1: NP_000927.1

RefSeq ID-2: NM_000936.4

Summary: PNLIP encodes a pancreatic lipase enzyme that facilitates the breakdown of dietary triglycerides in the small intestine, playing a key role in fat digestion. Experimental inhibition of PNLIP has been shown to prevent obesity induced by high-fat diets in mice and promote weight loss in humans with obesity. Mutations in this gene cause congenital pancreatic lipase deficiency, a rare disorder characterized by steatorrhea.

AdipoUpX™ Human PNLIP MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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