AdipoUpX™ Human AFF4 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human AFF4 MSCV Particle (Overexpression) is a powerful gene delivery tool designed for the efficient, stable expression of the human AFF4 gene. As a scaffold protein in the super elongation complex, its stable integration is vital for researching the transcriptional regulation of adipocyte differentiation. The MSCV vector is optimized for hematopoietic stem cells, stem cells, and other difficult mammalian cell types, ensuring robust and sustained gene expression. All products undergo rigorous testing for titer, sterility, and mycoplasma to ensure they meet the highest research standards for reproducibility.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: AFF4

Full Name: ALF transcription elongation factor 4

Gene Symbol: MCEF; CHOPS; AF5Q31

Gene ID: 27125

RefSeq ID-1: NP_055238.1

RefSeq ID-2: NM_014423.4

Summary: AFF4 encodes a transcription-associated factor that belongs to the AF4 protein family and serves as a component of the positive transcription elongation factor b (P-TEFb) complex. Through its role in regulating transcriptional elongation, AFF4 contributes to the control of gene expression programs that govern cell growth and differentiation. Chromosomal rearrangements involving AFF4 and the MLL gene on chromosome 11 have been identified in cases of infant acute lymphoblastic leukemia, underscoring the gene’s importance in transcriptional regulation and disease etiology.

AdipoUpX™ Human AFF4 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

Related Products