Product Overview
Description:
AdipoUpX™ Human AGRP MSCV Particle (Overexpression) is a professionally engineered vector particle providing efficient and stable overexpression of human AGRP. AGRP is the primary orexigenic peptide that stimulates hunger by antagonizing MC4R. Its MSCV structure is optimized specifically for hematopoietic stem cells, stem cells, etc., providing stable integration to study the central regulation of feeding behavior in stable cell models. All products undergo rigorous testing to ensure quality and efficacy, supporting rapid validation of appetite pathways.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
AGRP
Full Name:
Agouti related neuropeptide
Gene Symbol:
ART; AGRT; ASIP2
Summary:
AGRP codes for an extremely potent neuropeptide produced in the hypothalamus. It functions as a powerful inverse agonist that competitively blocks the Melanocortin-4 receptor (MC4R), the brain's main satiety center. By interfering with the MC4R's ability to receive appetite-suppressing signals, AGRP effectively promotes feeding behavior and reduces energy expenditure. This powerful, sustained antagonism is a primary mechanism for hypothalamic control over weight homeostasis. Gene mutations in AGRP are definitively associated with various forms of late-onset obesity.
